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rapamycin complex 2 mtorc2 activity  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rapamycin complex 2 mtorc2 activity
    Western blotting for markers of the Pl3k/AKT/MTOR pathway of the ipsilateral hippocampus in sham and HI rat pups treated with vehicle or sildenafil. Median with individual data points representation, with cropped representative western blots. Full-length blots are presented in . ( A ) p-AKT, ( B ) p-MTOR ser 2448 (mTORC1), and ( C ) p-MTOR ser 2481 <t>(mTORC2).</t> Significance: p value from Kruskal–Wallis test, with Dunn’s post-hoc comparison tests * p < 0.05, ** p < 0.01. Number of animals: n = 6/8/7 sham vehicle, 6/8/7 HI vehicle, and 7/8/10 HI 50 mg/kg, respectively at P12/P17/P30.
    Rapamycin Complex 2 Mtorc2 Activity, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1775 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rapamycin+complex+2+mtorc2+activity/Rapamycin/pmc08586032-219-83-98
    Average 96 stars, based on 1775 article reviews
    rapamycin complex 2 mtorc2 activity - by Bioz Stars, 2026-11
    96/100 stars

    Images

    1) Product Images from "Sildenafil improves hippocampal brain injuries and restores neuronal development after neonatal hypoxia–ischemia in male rat pups"

    Article Title: Sildenafil improves hippocampal brain injuries and restores neuronal development after neonatal hypoxia–ischemia in male rat pups

    Journal: Scientific Reports

    doi: 10.1038/s41598-021-01097-6

    Western blotting for markers of the Pl3k/AKT/MTOR pathway of the ipsilateral hippocampus in sham and HI rat pups treated with vehicle or sildenafil. Median with individual data points representation, with cropped representative western blots. Full-length blots are presented in . ( A ) p-AKT, ( B ) p-MTOR ser 2448 (mTORC1), and ( C ) p-MTOR ser 2481 (mTORC2). Significance: p value from Kruskal–Wallis test, with Dunn’s post-hoc comparison tests * p < 0.05, ** p < 0.01. Number of animals: n = 6/8/7 sham vehicle, 6/8/7 HI vehicle, and 7/8/10 HI 50 mg/kg, respectively at P12/P17/P30.
    Figure Legend Snippet: Western blotting for markers of the Pl3k/AKT/MTOR pathway of the ipsilateral hippocampus in sham and HI rat pups treated with vehicle or sildenafil. Median with individual data points representation, with cropped representative western blots. Full-length blots are presented in . ( A ) p-AKT, ( B ) p-MTOR ser 2448 (mTORC1), and ( C ) p-MTOR ser 2481 (mTORC2). Significance: p value from Kruskal–Wallis test, with Dunn’s post-hoc comparison tests * p < 0.05, ** p < 0.01. Number of animals: n = 6/8/7 sham vehicle, 6/8/7 HI vehicle, and 7/8/10 HI 50 mg/kg, respectively at P12/P17/P30.

    Techniques Used: Western Blot, Comparison

    Related Articles

    Activity Assay:

    Article Title: Sildenafil improves hippocampal brain injuries and restores neuronal development after neonatal hypoxia–ischemia in male rat pups
    Article Snippet: To determine whether the neuronal changes translated to the axonal level, we also measured the levels of the anti-growth associated protein 43 (GAP-43) (Sheep anti-GAP-43, NBP1-41123; Novus Biologicals, Centennial, Colorado, USA; dilution 1:1000), a protein expressed at high levels in neuronal growth cones during neuronal development and also highly expressed during axonal regeneration. .. To evaluate the activity of the Pl3k/AKT/mTOR pathway, we quantified the levels of phosphorylated protein kinase B (AKT) (rabbit anti-phospho-Akt Ser473 D9E, 4060; Cell Signaling Technology, Danvers, Massachusetts, USA; dilution 1:1000); the levels of phospho-mTOR serine 2448 (rabbit anti-p-mTOR Ser2448, 2971; Cell Signaling Technology, Danvers, Massachusetts, USA; dilution 1:1000), which reflect the mammalian target of rapamycin complex 1 (mTORC1) activity; the levels of phospho-mTOR Ser2481 (rabbit anti-p-mTOR Ser2481, 2974; Cell Signaling Technology, Danvers, Massachusetts, USA; dilution 1:1000), which reflect the mammalian target of rapamycin complex 2 (mTORC2) activity; and the levels of pan mTOR antibody (rabbit anti-mTOR, 2972; Cell Signaling Technology, Danvers, Massachusetts, USA; dilution 1:1000). ..



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    rapamycin complex 2 mtorc2 activity  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc rapamycin complex 2 mtorc2 activity
    Western blotting for markers of the Pl3k/AKT/MTOR pathway of the ipsilateral hippocampus in sham and HI rat pups treated with vehicle or sildenafil. Median with individual data points representation, with cropped representative western blots. Full-length blots are presented in . ( A ) p-AKT, ( B ) p-MTOR ser 2448 (mTORC1), and ( C ) p-MTOR ser 2481 <t>(mTORC2).</t> Significance: p value from Kruskal–Wallis test, with Dunn’s post-hoc comparison tests * p < 0.05, ** p < 0.01. Number of animals: n = 6/8/7 sham vehicle, 6/8/7 HI vehicle, and 7/8/10 HI 50 mg/kg, respectively at P12/P17/P30.
    Rapamycin Complex 2 Mtorc2 Activity, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rapamycin+complex+2+mtorc2+activity/Rapamycin/pmc08586032-219-83-98
    Average 96 stars, based on 1 article reviews
    rapamycin complex 2 mtorc2 activity - by Bioz Stars, 2026-11
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Western blotting for markers of the Pl3k/AKT/MTOR pathway of the ipsilateral hippocampus in sham and HI rat pups treated with vehicle or sildenafil. Median with individual data points representation, with cropped representative western blots. Full-length blots are presented in . ( A ) p-AKT, ( B ) p-MTOR ser 2448 (mTORC1), and ( C ) p-MTOR ser 2481 (mTORC2). Significance: p value from Kruskal–Wallis test, with Dunn’s post-hoc comparison tests * p < 0.05, ** p < 0.01. Number of animals: n = 6/8/7 sham vehicle, 6/8/7 HI vehicle, and 7/8/10 HI 50 mg/kg, respectively at P12/P17/P30.

    Journal: Scientific Reports

    Article Title: Sildenafil improves hippocampal brain injuries and restores neuronal development after neonatal hypoxia–ischemia in male rat pups

    doi: 10.1038/s41598-021-01097-6

    Figure Lengend Snippet: Western blotting for markers of the Pl3k/AKT/MTOR pathway of the ipsilateral hippocampus in sham and HI rat pups treated with vehicle or sildenafil. Median with individual data points representation, with cropped representative western blots. Full-length blots are presented in . ( A ) p-AKT, ( B ) p-MTOR ser 2448 (mTORC1), and ( C ) p-MTOR ser 2481 (mTORC2). Significance: p value from Kruskal–Wallis test, with Dunn’s post-hoc comparison tests * p < 0.05, ** p < 0.01. Number of animals: n = 6/8/7 sham vehicle, 6/8/7 HI vehicle, and 7/8/10 HI 50 mg/kg, respectively at P12/P17/P30.

    Article Snippet: To evaluate the activity of the Pl3k/AKT/mTOR pathway, we quantified the levels of phosphorylated protein kinase B (AKT) (rabbit anti-phospho-Akt Ser473 D9E, 4060; Cell Signaling Technology, Danvers, Massachusetts, USA; dilution 1:1000); the levels of phospho-mTOR serine 2448 (rabbit anti-p-mTOR Ser2448, 2971; Cell Signaling Technology, Danvers, Massachusetts, USA; dilution 1:1000), which reflect the mammalian target of rapamycin complex 1 (mTORC1) activity; the levels of phospho-mTOR Ser2481 (rabbit anti-p-mTOR Ser2481, 2974; Cell Signaling Technology, Danvers, Massachusetts, USA; dilution 1:1000), which reflect the mammalian target of rapamycin complex 2 (mTORC2) activity; and the levels of pan mTOR antibody (rabbit anti-mTOR, 2972; Cell Signaling Technology, Danvers, Massachusetts, USA; dilution 1:1000).

    Techniques: Western Blot, Comparison